An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes
Identifieur interne : 000D68 ( Main/Exploration ); précédent : 000D67; suivant : 000D69An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes
Auteurs : Laila Ali Layqah ; Shimaa EissaSource :
- Mikrochimica Acta [ 0026-3672 ] ; 2019.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (métabolisme), Coronavirus du syndrome respiratoire du Moyen-Orient (), Dosage immunologique (), Hexacyanoferrates II (), Infections à coronavirus (diagnostic), Limite de détection, Nanoparticules métalliques (), Or (), Oxydoréduction, Propriétés de surface, Sensibilité et spécificité, Taille de particule, Techniques de biocapteur (), Techniques électrochimiques (), Électrodes.
- MESH :
- diagnostic : Infections à coronavirus.
- métabolisme : Anticorps antiviraux.
- Coronavirus du syndrome respiratoire du Moyen-Orient, Dosage immunologique, Hexacyanoferrates II, Limite de détection, Nanoparticules métalliques, Or, Oxydoréduction, Propriétés de surface, Sensibilité et spécificité, Taille de particule, Techniques de biocapteur, Techniques électrochimiques, Électrodes.
English descriptors
- KwdEn :
- Antibodies, Viral (metabolism), Biosensing Techniques (methods), Coronavirus Infections (diagnosis), Electrochemical Techniques (methods), Electrodes, Ferrocyanides (chemistry), Gold (chemistry), Immunoassay (methods), Limit of Detection, Metal Nanoparticles (chemistry), Middle East Respiratory Syndrome Coronavirus (chemistry), Oxidation-Reduction, Particle Size, Sensitivity and Specificity, Surface Properties.
- MESH :
- chemical , chemistry : Ferrocyanides, Gold.
- chemical , metabolism : Antibodies, Viral.
- chemistry : Metal Nanoparticles, Middle East Respiratory Syndrome Coronavirus.
- diagnosis : Coronavirus Infections.
- methods : Biosensing Techniques, Electrochemical Techniques, Immunoassay.
- Electrodes, Limit of Detection, Oxidation-Reduction, Particle Size, Sensitivity and Specificity, Surface Properties.
Abstract
The Middle East respiratory syndrome corona virus (MERS-CoV) is highly pathogenic. An immunosensor for the determination of MERS-CoV is described here. It is based on a competitive assay carried out on an array of carbon electrodes (DEP) modified with gold nanoparticles. Recombinant spike protein S1 was used as a biomarker for MERS CoV. The electrode array enables multiplexed detection of different CoVs. The biosensor is based on indirect competition between free virus in the sample and immobilized MERS-CoV protein for a fixed concentration of antibody added to the sample. Voltammetric response is detected by monitoring the change in the peak current (typically acquired at a working potential of −0.05 V vs. Ag/AgCl) after addition of different concentrations of antigen against MERS-CoV. Electrochemical measurements using ferrocyanide/ferricyanide as a probe were recorded using square wave voltammetry (SWV). Good linear response between the sensor response and the concentrations from 0.001 to 100 ng.mL−1 and 0.01 to 10,000 ng.mL−1 were observed for MERS-CoV and HCoV, respectively. The assay was performed in 20 min with detection limit as low as 0.4 and 1.0 pg.mL−1 for HCoV and MERS-CoV, respectively. The method is highly selective over non-specific proteins such as Influenza A and B. The method is single-step, sensitive and accurate. It was successfully applied to spiked nasal samples.
An electrochemical immunoassay is described for the Middle East Respiratory Syndrome Corona Virus (MERS-CoV). The method is based on a competitive assay carried out on a carbon array electrodes (DEP) nanostructured with gold nanoparticles. The array electrodes enable the multiplexed detection of different types of Corona Virus.
The online version of this article (10.1007/s00604-019-3345-5) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1007/s00604-019-3345-5
PubMed: 30847572
PubMed Central: 7088225
Affiliations:
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Le document en format XML
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id="Aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Mikrochimica Acta</title><idno type="ISSN">0026-3672</idno><idno type="eISSN">1436-5073</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (metabolism)</term><term>Biosensing Techniques (methods)</term><term>Coronavirus Infections (diagnosis)</term><term>Electrochemical Techniques (methods)</term><term>Electrodes</term><term>Ferrocyanides (chemistry)</term><term>Gold (chemistry)</term><term>Immunoassay (methods)</term><term>Limit of Detection</term><term>Metal Nanoparticles (chemistry)</term><term>Middle East Respiratory Syndrome Coronavirus (chemistry)</term><term>Oxidation-Reduction</term><term>Particle Size</term><term>Sensitivity and Specificity</term><term>Surface Properties</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (métabolisme)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient ()</term><term>Dosage immunologique ()</term><term>Hexacyanoferrates II ()</term><term>Infections à coronavirus (diagnostic)</term><term>Limite de détection</term><term>Nanoparticules métalliques ()</term><term>Or ()</term><term>Oxydoréduction</term><term>Propriétés de surface</term><term>Sensibilité et spécificité</term><term>Taille de particule</term><term>Techniques de biocapteur ()</term><term>Techniques électrochimiques ()</term><term>Électrodes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Ferrocyanides</term><term>Gold</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Metal Nanoparticles</term><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term><term>Electrochemical Techniques</term><term>Immunoassay</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electrodes</term><term>Limit of Detection</term><term>Oxidation-Reduction</term><term>Particle Size</term><term>Sensitivity and Specificity</term><term>Surface Properties</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Dosage immunologique</term><term>Hexacyanoferrates II</term><term>Limite de détection</term><term>Nanoparticules métalliques</term><term>Or</term><term>Oxydoréduction</term><term>Propriétés de surface</term><term>Sensibilité et spécificité</term><term>Taille de particule</term><term>Techniques de biocapteur</term><term>Techniques électrochimiques</term><term>Électrodes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">The Middle East respiratory syndrome corona virus (MERS-CoV) is highly pathogenic. An immunosensor for the determination of MERS-CoV is described here. It is based on a competitive assay carried out on an array of carbon electrodes (DEP) modified with gold nanoparticles. Recombinant spike protein S1 was used as a biomarker for MERS CoV. The electrode array enables multiplexed detection of different CoVs. The biosensor is based on indirect competition between free virus in the sample and immobilized MERS-CoV protein for a fixed concentration of antibody added to the sample. Voltammetric response is detected by monitoring the change in the peak current (typically acquired at a working potential of −0.05 V vs. Ag/AgCl) after addition of different concentrations of antigen against MERS-CoV. Electrochemical measurements using ferrocyanide/ferricyanide as a probe were recorded using square wave voltammetry (SWV). Good linear response between the sensor response and the concentrations from 0.001 to 100 ng.mL<sup>−1</sup> and 0.01 to 10,000 ng.mL<sup>−1</sup> were observed for MERS-CoV and HCoV, respectively. The assay was performed in 20 min with detection limit as low as 0.4 and 1.0 pg.mL<sup>−1</sup> for HCoV and MERS-CoV, respectively. The method is highly selective over non-specific proteins such as Influenza A and B. The method is single-step, sensitive and accurate. It was successfully applied to spiked nasal samples.</p><p id="Par2"><fig position="anchor" id="Figa"><label>Graphical abstract</label><caption><p>An electrochemical immunoassay is described for the Middle East Respiratory Syndrome Corona Virus (MERS-CoV). The method is based on a competitive assay carried out on a carbon array electrodes (DEP) nanostructured with gold nanoparticles. The array electrodes enable the multiplexed detection of different types of Corona Virus.</p></caption><graphic position="anchor" xlink:href="604_2019_3345_Figa_HTML" id="MO1"></graphic></fig></p><sec><title>Electronic supplementary material</title><p>The online version of this article (10.1007/s00604-019-3345-5) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Hamre, D" uniqKey="Hamre D">D Hamre</name></author><author><name sortKey="Procknow, Jj" uniqKey="Procknow J">JJ Procknow</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name></author><author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name></author><author><name sortKey="Kang, Y" uniqKey="Kang Y">Y Kang</name></author><author><name sortKey="Chen, H" uniqKey="Chen H">H Chen</name></author><author><name sortKey="Luk, Hk" 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